Skrining Fitokimia dan Penetapan Kadar Flavonoid Total Ekstrak Biji Kopi Robusta (Coffea canephora) asal Lampung Barat

Authors

  • Kiki Yuli Handayani Institut Teknologi Sumatera
  • Rudi Setiawan Institut Teknologi Sumatera
  • Agitha Casanova Capah Institut Teknologi Sumatera
  • Natasya Armelia Putri Institut Teknologi Sumatera
  • Peggy Ivana Prayuda Institut Teknologi Sumatera
  • Suci Fitria Institut Teknologi Sumatera

DOI:

https://doi.org/10.29408/sinteza.v6i1.31794

Keywords:

Robusta coffee, Coffee bean extract, Phytochemical Analysis, Flavonoid content, UV-Visible Spectrophotometry

Abstract

Robusta coffee (Coffea canephora) is one of Indonesia’s leading agricultural commodities, with West Lampung serving as a major production center. Besides its economic significance, robusta coffee has garnered scientific attention due to its potential as a source of bioactive compounds. Among these, flavonoids stand out for their well-documented antioxidant, anti-inflammatory, and anti-aging properties, making them highly relevant for applications in cosmetic and personal care products, particularly in hair care formulations. Flavonoids are known to play a crucial role in maintaining scalp health, preventing oxidative damage to hair follicles, and promoting overall hair vitality. This study aimed to investigate the phytochemical profile and quantify the total flavonoid content in Coffea canephora bean extract originating from West Lampung. Phytochemical screening was conducted qualitatively to detect the presence of secondary metabolites such as alkaloids, tannins, saponins, and flavonoids. For the quantification of total flavonoid content, a UV-Visible spectrophotometric method was employed, using quercetin as the standard reference compound. The extract was first macerated with 96% ethanol, followed by filtration and evaporation to obtain a concentrated sample. The results revealed the presence of key bioactive compounds, including tannins, saponins, and notably, flavonoids. The total flavonoid content was found to be 784 mg QE/g extract. These findings support the potential utilization of Coffea canephora from West Lampung as a functional raw material in cosmetic formulations, particularly for hair care products aimed at protecting against oxidative stress and enhancing scalp and hair health. Further studies are recommended to explore the extract’s mechanism of action and its compatibility in cosmetic bases.

References

Aisyah, N., Sunarti, T., & Meryandini, A. (2025). Antioxidant activity, total phenolic, flavonoid, and caffeine contents of robusta coffee (Coffea canephora) fermented with lactic acid bacteria. Menara Perkebunan, 9(1), 21-30. doi:https://doi.org/10.22302/iribb.jur.mp.v93i1.604

Akhmadi, C., Utami, W., & Annisaa, E. (2022). Senyawa Fitokimia dan Aktivitas Farmakologi Family Basellaceae sebagai Obat Luka : A Narrative Review. Generics: Journal of Research in Pharmacy, 2(2), 77-85. doi:https://doi.org/10.14710/genres.v2i2.13798

Aryantini, D. (2021). AKTIVITAS ANTIOKSIDAN dan KANDUNGAN TANIN TOTAL EKSTRAK ETANOL DAUN KUPU-KUPU (Bauhinia purpurea L.). Jurnal Farmagazine, 8(1), 54. doi:https://doi.org/10.47653/farm.v8i1.537

Badan Pusat Statistik. (2022). Statistik Perkebunan Indonesia: Kopi. Retrieved 12 15, 2024

Fibrianto, K., ‘Izza, A., Martati, E., & I. A. . (2023). The potentials of Robusta (Coffea Robusta L.) and Arabica (Coffea Arabica L.) coffee leaf by-product as anti-diabetic drinks. Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 154-166. doi:https://doi.org/10.20956/canrea.v6i2.974

Firda, Y. (2024). Review Artikel : Penggunaan Herbal Dalam Sediaan Shampo Penumbuh Rambut. Makassar Natural Product Journal (MNPJ), 2((2)). doi:https://doi.org/10.33096/mnpj.v2i2.160

Fitri, H. A., Maharani, A. E., & Suparman, S. (2024). Validasi metode dan penetapan kadar flavonoid total sediaan pembersih wajah berbasis rosella menggunakan spektrofotometri UV-Vis. Acta Pharmaceutica Indonesia, 49(2), 51-59. Retrieved from https://www.google.com/url?sa=t&source=web&rct=j&opi=89978449&url=https://journals.itb.ac.id/index.php/acta/article/download/24600/6899/88277&ved=2ahUKEwiU4NrR8P2RAxWsR2wGHfZ7BiAQFnoECBkQAQ&usg=AOvVaw0u2KxLUE7XmMxFW267ds19

Gunawan, D. (2018). Penurunan Senyawa Saponin pada Gel Lidah Buaya dengan Perebusan dan Pengukusan. Teknologi Pangan : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian, 9(1), 41-44. doi:https://doi.org/10.35891/tp.v9i1.938

Hasma, Yusnita, U., & Andi, N. P. (2023). Uji Fitokimia dan Stabilitas Fisik Sediaan Hair Tonic. Jurnal MIPA, 13((1)), 7-12. Retrieved from https://ejournal.unsrat.ac.id/v3/index.php/jmuo/index

Heloisa , T., Luana, A. P., Gabriel, S. V., Albert, K., Derielsen, B. S., Ronaldo, L. M., . . . Paula, C. P. (2024). Effects of geographical origin and post-harvesting processing on the bioactive compounds and sensory quality of Brazilian specialty coffee beans. Food Research International, 186. doi:https://doi.org/10.1016/j.foodres.2024.114346

Husnia, F., & Budiarti, A. (2021). PENGEMBANGAN METODE ANALISIS KUERSETIN DALAM EKSTRAK ETANOL BUAH LEUNCA (Solanum nigrum L.) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI. Media Farmasi, 17(2). doi:10.32382/mf.v17i2.2209

Koja, R., Prangdimurti, E., & Edi Giriwo, P. (2024). Utilization of Suji Leaves Extract (Pleomele Angustifolia N.E Brown) in Inhibiting Carrageenan-Induced Inflammation on Rats. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment, 42-49. doi:https://doi.org/10.29165/ajarcde.v8i1.371

Kurniawan, S. S. (2022). Utilization of Garcinia Mangostana L. Peel Extract Which Contains Tanin in Protecting Tofu Cake Protein. Majalah Ilmiah Peternakan, 25(1), 18. doi:https://doi.org/10.24843/MIP.2022.v25.i01.p04

Luvedika, M., Ladhurshika , N., S. P. N. N. , S., C. B. , R., A. K. , C., & R. N. , P. (2024). Phytochemical Testing Methodologies and Principles for Preliminary Screening/Qualitative Testing. Asian Plant Research Journal, 12(5), 11-38. doi:https://doi.org/10.9734/aprj/2024/v12i5267

Muangsanguan, A., Linsaenkart, P., Chaitep, T., Sangta, J., Sommano, S., & Ruksriwanich, W. (2023). Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction. Foods, 12(22), 4116. doi:https://doi.org/10.3390/foods12224116

Nadie, F., Rendra, C. P., Amandia , D. S., Nuzulul , H., Hafiedz, M., Dwi, K. A., . . . Hanna, A. P. (2025). Robusta Coffee Husk Extract Increases the Number of Fibroblast and Collagen Density in Gingival Rat Periodontitis. National Library of Medicine, 1-7. doi:10.1155/sci5/8952540

Nicolescu, A., Bunea, , C., & Mocan,, A. (2023). Digital database of absorption spectra of diverse flavonoids enables structural comparisons and quantitative evaluations. Molecules, 28(4), 1679. doi:https://doi.org/10.3390/molecules28041679

Ningsih, A. W., Nur Arifin, J. F., Devitri, R. W., Septiarini, R. T., Zahara, E. S., Maulidya, V., . . . Klau, I. S. (2025). Tinjauan Literatur: Efektivitas Maserasi sebagai Metode Ekstraksi Fitokimia. OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan, 3(6), 228-242. doi:https://doi.org/10.61132/obat.v3i6.1927

Ningsih, W. P., Widiastuti , R., & Eltivitasari, A. (2023). Formulasi dan Uji Karakteristik Fisik Sediaan Masker Clay Serbuk Biji Kopi Robusta (Coffea robusta). Sinteza, 3((1)), 1–8. doi:https://doi.org/10.29408/sinteza.v3i1.7427

Noor , A. F., & Fan, Z. (2023). Febrianto, N. A., [penulis lainnya]. (2023). Coffee bean processing: Emerging methods and their influence on volatile and phenolic compounds in Robusta coffee (Coffea canephora). Food Chemistry, 412, 1-20. doi:https://doi.org/10.1016/j.foodchem.2023.135489

Putri, M. I., & Anggraini, D. (2022). Flavonoid sebagai Antioksidan Alami: Struktur, Mekanisme Kerja dan Potensi Aplikasinya. Jurnal Kimia Riset, 10((1)), 56-66. doi:https://doi.org/10.35799/jkr.v10i1.345

Rialdi, S. (2021). STRATEGI PENGEMBANGAN KOPI ROBUSTA DI KABUPATEN MERANGIN. Jurnal Khazanah Intelektual, 4(3), 866–888. doi:https://doi.org/10.37250/newkiki.v4i3.79

Rosalina, Y., & Rinda, Y. K. (2021). Phytochemical Test and Antioxidant Activity of Methanol Extract in Arabica Coffee Leaves by Using DPPH Method (1,1-Diphenyl-2-Picrylhydrazyl). Walisongo Journal of Chemistry, 4((2)), 113-118. doi:https://doi.org/10.21580/wjc.v4i2.8032

Rosidah, U., Sugito, S., Yuliati, K., Abdiansyah, A., & Anggraini, F. (2021). Identifikasi Senyawa Fitokimia dan Aktivitas Antioksidan Minuman Fungsional Cascara dari Kulit Kopi dengan Fermentasi Terkendali. Prosiding Seminar Nasional Lahan Suboptimal ke-9 (pp. 611-620). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).

Sandeep, T. N., Channabasamma, B. B., Gopinandhan, T. N., & Nagaraja, J. S. (2021). The effect of drying temperature on cup quality of coffee subjected to mechanical drying. Journal of Plantation Crops, 49(1), 35–41. doi:http://dx.doi.org/10.25081/jpc.2021.v49.i1.7059

Shraim, A., Ahmed, T., Rahman, M., & Hijj. (2021). Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT - Food Science and Technology, 150. doi:https://doi.org/10.1016/j.lwt.2021.111932

Sokratis , E. K., Chrysanthos , S., Christoforos , V., & Magdalini , K. (2025). Sustainable Valorization of Coffee Silverskin Waste: Pressurized. Foods, 14(4), 615. doi:10.3390/foods14040615

Sulastri, E., S.Z, M., Anas, N., Abidin, S., Hardani, R., Yulianti, R., & Aliyah. (2018). Total Phenolic, Total Flavonoid, Quercetin Content and Antioxidant Activity of Standardized Extract of Moringa Oleifera Leaf from Regions with Different Elevation. Pharmacogn, 10(6).

Sultana, S., Hossain, L., Sostaric, T., Yong Lim, L., Foster, K., & Locher, C. (2024). Investigating Flavonoids by HPTLC Analysis Using Aluminium Chloride as Derivatization Reagent. Molecules, 29(21), 5161. doi:https://doi.org/10.3390/molecules29215161

Tadros, T. (2018). Formulation science and technology: Pharmaceutical, cosmetic, and personal care formulations. Walter de Gruyter.

Tandi, J., Riani, N., Handayani, T., & Kanan, M. (2023). Determination of secondary metabolites and antioxidant activity of robusta coffee bean ethanol extract by UV-Vis spectrophotometry. Jurnal Akademika Kimia, 12((1)), 58-64. doi:https://doi.org/10.22487/j24775185.2023.v12.i1.pp58-64

Utami, N. M., & Wulandari, D. (2023). Analisis Fitokimia dan Aktivitas Antimikroba Ekstrak Daun Kirinyuh. Jurnal Biologi Tropis, 21((3)), 187-195. doi:https://doi.org/10.25077/jbt.21.3.187-195.2023

Widyasari, R., Fadli, & Handayani, S. (2020). PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK METANOL KULIT JERUK SAMBAL SECARA SPEKTROFOTOMETRI UV-VISIBEL. Medical Sains, 4(2), 111-118.

Wigati, E., Pratiwi, E., Nissa, T., & N. F. U. (2019). UJI KARAKTERISTIK FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN BIJI KOPI ROBUSTA (Coffea canephora Pierre) DARI BOGOR, BANDUNG DAN GARUT DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl). FITOFARMAKA:Jurnal Ilmiah Farmasi, 8(1), 53-59. doi:https://doi.org/10.33751/jf.v8i1.1172

Yasir, A. S., Renita, A. Y., & Tutik, T. (2023). Evaluation and clinical activity test of various concentrations of peel-off gel mask of robusta coffee seed extract (Coffea canephora) as anti-aging. Pharmaciana, 13((1)), 87-99. doi:http://dx.doi.org/10.12928/pharmaciana.v13i1.23965

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Published

2026-02-10

How to Cite

Handayani, K. Y., Setiawan, R., Capah, A. C., Putri, N. A., Prayuda, P. I., & Fitria, S. (2026). Skrining Fitokimia dan Penetapan Kadar Flavonoid Total Ekstrak Biji Kopi Robusta (Coffea canephora) asal Lampung Barat . Sinteza, 6(1), 1–16. https://doi.org/10.29408/sinteza.v6i1.31794

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Vol. 6 No. 1 (2026): February

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