Formulasi dan Uji Aktivitas Antibakteri Sediaan Mouthwash Fraksi Metanol Daun Seledri (Apium graveolens L.) terhadap Pertumbuhan Bakteri Streptococcus mutans Penyebab Karies Gigi

Authors

  • Mirzalina Zulhijjati Universitas Hamzanwadi
  • Tri Puspita Yuliana Hamzanwadi
  • Baiq Risma Fatmayanti Universitas Hamzanwadi
  • Erma Ewisa Oktresia Universitas Hamzanwadi

DOI:

https://doi.org/10.29408/sinteza.v6i1.31994

Keywords:

Celery leaves, Streptococcus mutans, Mouthwash preparation

Abstract

Streptococcus mutans is a bacterium found in the human oral cavity and is the primary cause of tooth decay. The effects of Streptococcus mutans bacteria can be eliminated with mouthwash formulated with natural ingredients, one of which is celery leaves, which are known to have antibacterial properties against the growth of Streptococcus mutans. The purpose of this study was to determine the best concentration of a mouthwash formulation using the methanol fraction of celery leaves based on the results of the physical properties evaluation test and to determine the antibacterial activity of a mouthwash formulation using the methanol fraction of celery leaves in inhibiting the growth of Streptococcus mutans bacteria. This study was a quantitative study using a laboratory experimental method with a posttest-only control group design. This study is a quantitative study using a laboratory experiment method with a posttest-only control group design by observing the area of ​​the inhibition zone as the dependent variable. The area of ​​the inhibition zone of each formula was compared with the control using the ANOVA test. The results of the physical properties evaluation test, including ogrnanoleptic tests, pH tests, homogeneity tests, specific gravity tests, and cycling tests, met the requirements for good physical quality. The highest antibacterial activity results were found in formula III with a concentration of 1.5% celery leaf methanol fraction with an average inhibitory power of 23.63 mm with a Very Strong category.

References

Anastasia A, Yuliet, & Tandah MR. (2017). Mouthwash formulation of tooth plaque preventing of kakao (Theobroma cacao L) seed extract and effectivity test on Streptococcus mutans. Galenika Journal of Pharmacy, 3(1), 84–92.

Audies, A. (2015). Uji Efektivitas Antibakteri Ekstrak Kulit Nanas (Ananas comosus L.) Terhadap Pertumbuhan Bakteri Streptococcus mutans Penyebab Karies Gigi. Skripsi Fakultas Kedokteran Gigi Universitas Andalas.

Basuki, K. (2019). Hubungan Makanan Kariogenik dengan Kejadian Karies Gigi pada Anak Usia Prasekolah di PAUD Nusa Indah Desa Banjar Negeri Kecematan Lima Kabupaten Pasawaran. Repository Poltekes Denpasar, 53(9), 1689–1699. www.journal.uta45jakarta.ac.id

Constanty, I. C., & Tukiran, T. (2021). Aktivitas Antioksidan Dari Fraksi n-Heksana Kulit Batang Tumbuhan Jambu Semarang (Syzygium samarangense). Jurnal Kimia Riset, 6(1), 1. https://doi.org/10.20473/jkr.v6i1.24467

Dewi, I. P., Wijaya, W. R., & Verawaty. (2019). Uji Daya Hambat Deodoran Ekstrak Etanol Daun Kersen (Muntingia calabura L.) Terhadap Pertumbuhan Bakteri Staphylococcus epidermidis. Jurnal Akademi Farmasi Prayoga, 4(1), 19–28.

Dinda Izatul Waris, S. I. (2023). Formulasi Sabun Cair Cuci Tangan Dan Aktivitas Antibakteri Staphylococcus sureus Dari Ekstrak Daun Kelor (Moringa oleifera Lam.). Sinteza Journal Farmasi Klinis dan Sains Bahan Alam, 29-40.

Esterina, & Zuraida. (2017). Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Etanol 70% Daun Bangun-Bangun (Plectranthus Amboinicus (Lour.) Spreng.) Terhadap Bakteri Staphylococcus Aureus Dan Pseudomonas Aeruginosa. Indonesia Natural Research Pharmaceutical Journal, Vol 2, No, 59–66.

Fadillah Maryam, Burhanuddin Taebe, & Deby Putrianti Toding. (2020). Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun Matoa (Pometia pinnata J.R & G.Forst). Jurnal Mandala Pharmacon Indonesia, 6(1), 1–12.

Frastika, D. R. P. dan I. N. S. (2017). Uji Efektivitas Ekstrak Daun Kirinyuh (Chromolaena Odorata (L.) R. M. King Dan H. Rob) Sebagai Herbisida Alami Terhadap Perkecambahan Biji Kacang Hijau (Vigna Radiata (L.) R. Wilczek) Dan Biji Karuilei (Mimosa Invisa Mart. ex Colla). Natural Science: Journal of Science and Technology, 6(3), 225–238.

Handayani, F., Sundu, R., & Sari, R. M. (2017). Formulasi Dan Uji Aktivitas Antibakteri Streptococcus mutans Dari Sediaan Mouthwash Ekstrak Daun Jambu Biji ( Psidium guajava L .). Akademi Farmasi Samarinda, 1(8), 422–433.

Haryati, E., Pandanwangi TW, S., Nabila, E. R., & Nurpatmawati, N. (2023). Pembuatan Media Pertumbuhan Bakteri Menggunakan Tepung Biji Nangka (Artocarpus heterophyllus Lamk) Untuk Pertumbuhan Bakteri Staphylococcus aureus. PRAEPARANDI : Jurnal Farmasi Dan Sains, 7(1), 1. https://doi.org/10.58365/ojs.v7i1.215

Haryoto, & Putri, S. P. (2019). Aktivitas Sitotoksik Ekstrak Etanol, Fraksi Heksan, Etil Asetat dan Etanol-Air dari Daun Mangrove Tancang (Bruguiera gymnorrhiza) terhadap Sel Kanker Payudara T47D. Coastal And Estuarine Processes, 2018, 177–183.

Karim, S. F., Jumardin, W., Senolinggi, T., & Article, I. (2023). Formulasi Dan Uji Aktivitas Antibakteri Sediaan Mouthwash Fraksi Metanol Daun Pucuk Merah ( Syzygium myrtifolium , Walp .) Terhadap Bakteri Streptococcus mutans. Https://Ejournal.Unisba.Ac.Id/Index.Php/Farmasyifa/Article/View/11720, 6(2), 161–171. https://ejournal.unisba.ac.id/index.php/Farmasyifa/article/view/11720

Kemenkes RI. (2023). Survei Kesehatan Indonesia 2023 (SKI). Kemenkes, 235.

Kurniasih, E., Sari, M. P., & Febriyanti, R. (2022). Pengaruh Perbedaan Konsentrasi Propilenglikol Pada Uji Sifat Fisik Sediaan Deodoran Spray Ekstrak Daun Sirih (Piper betle L.). Jurnal Farmasi Politeknik Harapan Bersama Tegal, 5(2), 1–8.

Lianah, W., Ayuwardani, N., & Hariningsih, Y. (2021). Aktivitas Antibakteri Ekstrak Etanol Seledri (Apium graveolens L) Terhadap Pertumbuhan Bakteri Actinomyces sp. dan Lactobacillus acidophilus. Duta Pharma Journal, 1(1), 32–39. https://doi.org/10.47701/djp.v1i1.1190

Noval, N., Melviani, M., Novia, N., & Syahrina, D. (2020). Formulasi Dan Evaluasi Sediaan Obat Kumur (Mouthwash) Dari Ekstrak Etanol Tanaman Bundung (Actinoscirpus Grossus) Sebagai Antiseptik Mulut. Jurnal Surya Medika, 6(1), 112–120. https://doi.org/10.33084/jsm.v6i1.1626

RISKESDAS. (2018). Hasil Riset Kesehatan Dasar Tahun 2018. Kementrian Kesehatan RI, 53(9), 1689–1699.

Sakinah, N., Dwyana, Z., Tambaru, E., & Rante, H. (2015). Uji Aktivitas Sediaan Obat Kumur Ekstrak Daun Miana Coleus scutellarioides (L.) Benth Terhadap Pertumbuhan Bakteri Streptococcus mutans. Jurusan Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam, 1–7. https://core.ac.uk/reader/77626727

Sinta, P. H., Furtuna, D. K., & Fatmaria, F. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol 96% Umbi Bawang Suna (Allium schoenoprasum L.) Terhadap Pertumbuhan Staphylococcus aureus DAN Staphylococcus saprophyticus Dengan Metode Difusi Cakram Kirby-Bauer. Herb-Medicine Journal, 3(2), 7. https://doi.org/10.30595/hmj.v3i2.6379

Suryani, Y., L.W.Sophia, Cahyanto, T., & Kinasih, I. (2015). Uji Aktivitas Antibakteri Dan Antioksidan Infusum Cacing Tanah (Lumbricus rubellus) Dengan Tambahan Kitosan Udang Pada Salmonella typhi. Jurnal ISTEK, 9(2), 264–281.

Suwito, M. B. ; M. R. W. dan S. U. (2017). Efektivitas Ekstrak Seledri (Apium graveolens L. var. secalinum Alef.) Terhadap Pertumbuhan Bakteri Streptococcus mutans Sebagai Alternatif Obat Kumur. Jurnal Kedokteran Syiah Kuala, Volume 17, 159–163.

Utomo, S. B., Fujiyanti, M., Lestari, W. P., & Mulyani, S. (2018). Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria. JKPK (Jurnal Kimia Dan Pendidikan Kimia), 3(3), 201. https://doi.org/10.20961/jkpk.v3i3.22742

Wardaningrum, R. Y; Susilo, J. & D. (2019). Perbandingan Aktivitas Antioksidan Ekstrak Etanol Terpurifikasi Ubi Jalar Ungu (Ipomoea batatas L.) dengan Vitamin E. Program Studi Farmasi, Fakultas Ilmu Kesehatan. Ungaran: Universitas Ngudi Waluyo, 15.

Yasir, A. S., Rai Saputri, G. A., & Chandra, Y. (2020). Formulasi Sediaan Kumur Ekstrak Etanol 96% Daun Seledri (Apium graveolens L.) Sebagai Antibakteri Streptococcus mutans Penyebab Bau Mulut. Jurnal Farmasi Malahayati, 3(1), 1–11. https://doi.org/10.33024/jfm.v3i1.2433

Downloads

Published

2026-02-10

How to Cite

Zulhijjati, M., Yuliana, T. P., Fatmayanti, B. R., & Oktresia, E. E. (2026). Formulasi dan Uji Aktivitas Antibakteri Sediaan Mouthwash Fraksi Metanol Daun Seledri (Apium graveolens L.) terhadap Pertumbuhan Bakteri Streptococcus mutans Penyebab Karies Gigi. Sinteza, 6(1), 73–84. https://doi.org/10.29408/sinteza.v6i1.31994

Issue

Vol. 6 No. 1 (2026): February

Section

Articles

License

Copyright (c) 2026 Sinteza

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish in Sinteza, agree to retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.