ISOLASI DAN KARAKTERISTIK BAKTERI XILANOLITIK YANG BERPOTENSI SEBAGAI BIO-DEKOMPOSER
DOI:
https://doi.org/10.29408/cob.v9i2.27512Keywords:
bakteri, tebu, xylanaseAbstract
Sugarcane (Saccharum officinarum L.) is a plant used for sugar production with waste products in the form of bagasse and sugarcane leaves. Sugarcane pulp and leaves are known t°Contain hemicellulose (xylan) which has the potential to be used as a rough substrate for xylanase-producing microorganisms. The aim of the research was to isolate xylanolytic bacteria from sugar cane plantations and to know the characteristics of xylanolytic bacteria. The research was conducted in December 2022- February 2023. This research was divided into 2 parts, namely experiment and observation. Experimental research in the form of biological characters using Completely Randomized Factorial Design (RALF). The first factor was the pH of the media, namely pH 4, pH 5, and pH 6. The second factor was temperature, using a temperature of 25°C and 45°C. Each treatment was carried out with 3 replications. To determine the characteristics of xylanolytic bacteria, an observational study was carried out by conducting a hypersensitivity test and observing the macroscopic and microscopic morphology of the bacterial colonies. The results showed that the best bacterial pH in producing the enzymatic index was at pH 4 and 25°C. Hypersensitivity test showed that all isolates were pathogenic.
References
Herdiyantoro, D., M. R. Setiawati, T. Simarmata. (2022). Reaksi Hipersensitif Daun Tembakau oleh Isolat Bakteri Pelarut Kalium pada Praformulasi Pupuk Hayati. Soilrens. 20(2).
Inayah,Mazidah Noer. (2016). Karakterisasi Xilanase Dari Bakteri Xilanolitik Asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia. Skripsi. Institut Pertanian Bogor.
Lenni Fitri, Y. Yasmin. (2011). Isolasi Dan Pengamatan Morfologi Koloni Bakteri Kitinolitik. Jurnal Ilmiah Pendidikan Biologi.3(2):20-25.
Mandal. A. (2015). Review on microbial Xilanases and their applications. International Journal of Life Sciences. 4.(3):178-187.
Moodley, Preshanthan, E.B. Gueguim Kana. (2017). Comparative Study Of Three
Optimized Acid-Based Pretreatments For Sugar Recovery From Sugarcane Leaf Waste: A Sustainable Feedst°Ck For Biohydrogen Production. Engineering Science and Technology,an International Journal. Vol. 30.
Motta F.L., Andrade C.C.P., Santana M.H.A. (2013). A review of Xilanase production by the fermentation of Xilan: classification, characterization andapplications. In: Chandel A.K., da Silva S.S. (Eds) Sustainable Degradation of Lign°Cellulosic Biomass- Techniques, Applications and Commercialization. InTech. Croatia.
Oktari A, Y Supriatin, M Kamal, H. Syafrullah. (2017). The Bacterial Endospore Stain on Schaeffer Fulton using Variation of Methylene Blue Solution. Journal of Physics: Conf. Series 812 (2017) 012066.
Rahmayuni, E., S. Ismiani, D.H. Muslimah, E.D.I. Wilujeng, dan M.N. Rizqulloh. (2018). Karakterisasi dan viabilitas isolat bakteri pelarut fosfat dalam bahan pembawa kompos dan zeolit. Jurnal Agrosains dan Teknologi 3 (1): 31-38.
Seo. J. K., T. S. Park., I. H. Kwon., M. Y. Piao., C. H. Lee., J.K. Ha. (2013). Characterization Of Cellulolytic And Xylanolytic Enzymes Of Bacillus Licheniformis JK7 Isolated From The Rumen Of A Native Korean Goat. Asian-Aust. Jurnal Anim.Sci.26:50-58.
Sumardi, D. Lengkana. (2010). Isolasi Bacillus Penghasil Protease Dari Saluran Pencernaan Ayam Kampung. FMIPA Universitas Lampung. Hal. 5.
Trikuntari, D., Permadhi, D., & Putra, L. K. (2020). Analisis kinerja dan prospek komoditas gula. Opini dan Analisis Perkebunan.1(1):1-10.
Tuntun Maria, M. Huda. (2014). Isolasi Dan Identifikasi Bakteri Termofilik Dari Sumber Air Panas Way Panas Bumi Natar Lampung Selatan. Jurnal Analis Kesehatan. 3(1): 297- 304.
Yoon, J.H., Park, J.E., Suh, D.Y., Hong, S.B., Ko, S.J, Kim, S.H. (2007). Comparison of dyes for easy detection of extracellular cellulases in fungi. Mycobiology. 35(1):21 24.
Erika, R. Agustrina., Sumardi, Mulyono. (2015). Optimasi Media Produksi Xilanase Dari Bacillus sp. Jurnal Selulosa, 6(1):19-26.
Lestari P., W. Sarmiad., N. Richana.,S. Salma., Y. Suryadi. (2014). Evaluation of Cellulase Activity from Trichoderma spp.and Xylanolytic Bacteria. Jurnal Agrobiotech. 5: 1-1.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
